Lower GI Cancers

Archetype A 80 regimens (Main Regimens) lower_gi

Colon and rectal cancer — adjuvant and metastatic

DefinitionClick to collapse

Rectal cancer is a malignant epithelial neoplasm, most commonly adenocarcinoma, arising from the mucosal lining of the rectum. The rectum is anatomically defined as the portion of the large bowel lying below the peritoneal reflection, extending from the sacral promontory to the upper edge of the symphysis pubis as determined by magnetic resonance imaging (MRI) [REC-2 footnote k]. The rectum terminates at the superior border of the functional anal canal, defined as the palpable upper border of the anal sphincter and puborectalis muscles of the anorectal ring [MS-8]. The rectum is approximately 15 cm (6 inches) in length [MS-2].

Rectal cancer is staged using the American Joint Committee on Cancer (AJCC) Cancer Staging Manual, 8th Edition [ST-1, ST-2]. The TNM classification defines: T1 tumors invade the submucosa (through muscularis mucosae but not into muscularis propria); T2 tumors invade the muscularis propria; T3 tumors penetrate through the muscularis propria; T4a tumors directly penetrate to the surface of the visceral peritoneum; and T4b tumors directly invade or are adherent to other organs or structures. Regional lymph node classification includes N1a (1 positive node), N1b (2–3 positive nodes), N1c (tumor deposits without nodal metastasis), N2a (4–6 positive nodes), and N2b (≥7 positive nodes). Distant metastasis is classified as M0 (none) or M1 (present), with M1a (single distant site), M1b (multiple distant sites), and M1c (peritoneal surface metastasis) [ST-1].

The most common histologic type of rectal cancer is adenocarcinoma. Mucinous histology is also recognized and may have implications for imaging assessment [REC-A 3 of 5]. Molecular profiling has identified distinct biologic subtypes that dictate treatment approaches: proficient mismatch repair/microsatellite stable (pMMR/MSS) tumors, which represent the majority; deficient mismatch repair/microsatellite instability-high (dMMR/MSI-H) tumors; and tumors harboring polymerase proofreading domain mutations (POLE/POLD1) with ultra-hypermutated phenotype (tumor mutational burden [TMB] >50 mutations/megabase) [REC-2A, REC-B 7].

Rectal cancer differs from colon cancer in several critical respects: the rectum lacks a serosal covering, is in close proximity to pelvic structures and organs, presents technical challenges in achieving adequate surgical margins, and carries a higher risk of locoregional recurrence. These characteristics necessitate a distinct management approach that frequently incorporates neoadjuvant radiation therapy and total mesorectal excision (TME) [REC-C 1, REC-E]. The NCCN Guidelines for Rectal Cancer provide separate, disease-specific recommendations reflecting these unique considerations. T stage has more prognostic value than N stage in rectal cancer: patients with stage IIIA disease (T1–2) have longer rectal cancer-specific survival than patients with stage IIA (T3), IIB (T4a), or IIC (T4b) disease [MS-3].

EpidemiologyClick to collapse

Colorectal cancer (CRC) is the fourth most frequently diagnosed cancer and the second leading cause of cancer death in the United States [MS-2]. In 2025, an estimated 46,950 new cases of rectal cancer will occur in the United States (27,950 cases in males; 19,000 cases in females) [MS-2]. Approximately one-third of all colorectal cancers occur in the rectum [MS-2]. The overall age-adjusted incidence of colorectal cancer per 100,000 people decreased from 60.5 in 1976 to 46.4 in 2005 and, more recently, to 38.7 in 2016 [MS-2]. This decreasing overall trend reflects the impact of screening, polypectomy, and improved treatment, though significant increases are now noted in younger populations.
Annual Incidence
In 2025, it is estimated that 52,900 people will die from rectal and colon cancers combined in the United States [MS-2]. Mortality from colorectal cancer has been decreasing for decades (since 1947 in females and since 1980 in males) and is currently down by more than 50% from peak mortality rates [MS-2]. The decline in mortality is attributed to cancer prevention, earlier diagnoses through screening, and improved treatment modalities [MS-2]. More than half of patients who die of CRC have liver metastases at autopsy, with metastatic liver disease as the cause of death in most patients [MS-33].
Annual Mortality
Recent data show continued rapid declines in colorectal cancer incidence among those aged 65 years and older, with a decrease of 3.3% annually between 2011 and 2016 [MS-2]. Conversely, incidence has increased among those younger than 65 years, with a 1% annual increase in those aged 50 to 64 years and a 2% annual increase in those younger than 50 years [MS-2]. Mortality trends mirror these age-dependent patterns: declining by 3% annually for those 65 years and older, compared to a 0.6% annual decline for individuals aged 50 to 64 years and a 1.3% annual increase for individuals younger than 50 years [MS-2]. A retrospective cohort study of the SEER CRC registry estimated that incidence rates for rectal cancer will increase by 124.2% for patients aged 20 to 34 years by 2030 [MS-2]. CRC that occurs in patients younger than 45 years may be clinicopathologically and genetically different from CRC in older adults, though this has not been confirmed broadly [MS-2].
Trend & Projections
Colorectal cancer incidence and mortality rates vary by race and ethnicity, with the highest rates in non-Hispanic Black individuals and the lowest in Asian Americans/Pacific Islanders [MS-2]. The magnitude of disparity in mortality rates is double that of incidence rates [MS-2]. Reasons for these racial disparities include differences in risk factor prevalence, access to health care and other social determinants of health, comorbidities, and tumor characteristics [MS-2]. Approximately 20% of cases of colorectal cancer are associated with familial clustering, and first-degree relatives of patients with colorectal adenomas or invasive CRC are at increased risk [MS-3]. Metastatic disease develops in 50%–60% of patients diagnosed with CRC, with 20%–34% presenting with synchronous liver metastases at diagnosis [MS-33]. Approximately 17% of patients with metastatic CRC have peritoneal carcinomatosis, with 2% having the peritoneum as the only site of metastasis [MS-34]. Lung metastases occur in approximately 4%–9% of patients with colon and rectal cancer [MS-33].
Demographics

SubtypesClick to collapse

Majority of rectal cancers; the document addresses pMMR/MSS as the predominant subtype with distinct treatment pathways from dMMR/MSI-H and POLE/POLD1-mutated tumors
Proficient Mismatch Repair/Microsatellite Stable (pMMR/MSS) Adenocarcinoma

pMMR/MSS tumors exhibit intact DNA mismatch repair protein function and stable microsatellite regions, representing the predominant molecular subtype of rectal cancer. These tumors are characterized by a relatively lower mutational burden and follow the chromosomal instability pathway of colorectal carcinogenesis. The NCCN Guidelines provide extensive treatment recommendations stratified by specific biomarkers within this subtype, including KRAS, NRAS, and BRAF mutation status, as well as HER2 overexpression/amplification status. For locally advanced disease (stage II–III), the recommended approach is total neoadjuvant therapy (TNT) with either induction or consolidation chemotherapy combined with radiation therapy, followed by total mesorectal excision (TME) [REC-6]. For metastatic disease, treatment follows a continuum-of-care paradigm with multiple lines of systemic therapy, including fluoropyrimidine-based chemotherapy, oxaliplatin, irinotecan, anti-VEGF biologics (bevacizumab, ziv-aflibercept, ramucirumab), and anti-EGFR biologics (cetuximab, panitumumab) for RAS/BRAF wild-type tumors [REC-F 2–4].

Not specifically quantified for rectal cancer in this document; universal MMR/MSI testing is recommended for all newly diagnosed patients to identify this subtype and assess Lynch syndrome risk
Deficient Mismatch Repair/Microsatellite Instability-High (dMMR/MSI-H) Adenocarcinoma

dMMR/MSI-H rectal cancers are characterized by loss of function in one or more mismatch repair proteins (MLH1, MSH2, MSH6, PMS2), leading to microsatellite instability and a high mutational burden. MMR proteins function as heterodimers (MLH1/PMS2 and MSH2/MSH6), so loss of expression of one protein typically results in loss of its heterodimer partner [REC-B 6]. Universal MMR or MSI testing is recommended in all newly diagnosed rectal cancer patients [REC-B 5]. Loss of MLH1 expression in the setting of BRAF V600E mutation would preclude Lynch syndrome diagnosis in the vast majority of patients, though approximately 1% of cancers with BRAF V600E mutations and loss of MLH1 still harbor Lynch syndrome [REC-B 5]. These tumors demonstrate exceptional sensitivity to immune checkpoint inhibitor immunotherapy, including PD-1 inhibitors (nivolumab, pembrolizumab, dostarlimab-gxly, cemiplimab-rwlc, and others) and combination PD-1/CTLA-4 blockade (ipilimumab + nivolumab) [REC-F 5]. For locally advanced nonmetastatic disease, neoadjuvant immunotherapy with checkpoint inhibitors has achieved complete clinical response rates enabling organ preservation without surgery, chemotherapy, or radiation in many patients [REC-14].

Somatic POLE mutations occur in approximately 2–8% of patients with predominantly MSS/pMMR colorectal cancers; somatic POLD1 mutations are extremely rare [REC-B 7]
POLE/POLD1 Ultra-Hypermutated Adenocarcinoma

Tumors harboring somatic pathogenic variants in the exonuclease domains of POLE or POLD1 polymerase genes exhibit an ultra-hypermutated phenotype with TMB exceeding 50 mutations per megabase [REC-B 7]. POLE encodes the catalytic subunit of DNA polymerase epsilon, and POLD1 encodes the catalytic subunit of DNA polymerase delta, both involved in DNA replication proofreading. Loss of proofreading function leads to acquisition of numerous single nucleotide variants [REC-B 7]. Germline pathogenic variants predispose patients to polymerase proofreading-associated polyposis (PPAP) [REC-B 7]. These tumors demonstrate enhanced immune responses stimulated by numerous neoantigens, resulting in more favorable prognosis and excellent response to immune checkpoint inhibitor therapy [REC-B 7]. Multigene panel testing (MGPT) can identify POLE/POLD1 mutations and provides direct evidence of the ultra-hypermutated phenotype through TMB calculation [REC-B 7]. When both dMMR/MSI-H and BRAF V600E are positive, treatment should start with a checkpoint inhibitor if feasible [REC-F note].

Not specifically quantified in this document
Mucinous Adenocarcinoma

Mucinous adenocarcinoma is a histologic variant of rectal cancer characterized by the presence of extracellular mucin comprising more than 50% of the tumor volume. On pelvic MRI, mucinous tumors may demonstrate signal characteristics similar to fat on T2-weighted images, and T1-weighted images without contrast may be helpful for their identification and staging [REC-A 3 of 5]. This histologic subtype may have implications for imaging assessment and treatment response evaluation. The molecular profile and treatment approach generally follow the same guidelines as non-mucinous adenocarcinoma, contingent on MMR/MSI status and other biomarker testing results. For staging and restaging purposes, MRI assessment should account for the distinct signal characteristics of mucinous tumors.

Molecular PathogenesisClick to collapse

Rectal cancer molecular pathogenesis encompasses multiple genomic events, signaling pathways, and chromosomal abnormalities that define distinct biologic subtypes and guide therapeutic decision-making.

RAS/RAF Pathway: KRAS and NRAS mutations (affecting exons 2, 3, and 4) are key oncogenic drivers in colorectal cancer. Patients with any known KRAS or NRAS mutation should not receive anti-EGFR therapy with cetuximab or panitumumab, as these mutations confer resistance, unless given as part of a regimen targeting a KRAS G12C mutation [REC-B 5]. BRAF V600E mutation renders response to panitumumab or cetuximab highly unlikely [REC-B 5]. BRAF V600E mutation testing via immunohistochemistry (IHC) is an option [REC-B 5]. Encorafenib-based regimens (combined with cetuximab or panitumumab, with or without FOLFIRI or FOLFOX) are specifically indicated for BRAF V600E-mutant metastatic disease [REC-F 4]. Patients with BRAF mutations other than V600E may be considered for anti-EGFR therapy [REC-F footnote c].

Mismatch Repair/Microsatellite Instability: Deficient mismatch repair (dMMR) results from loss of function in MLH1, MSH2, MSH6, or PMS2 proteins. MMR proteins function as heterodimers (MLH1/PMS2 and MSH2/MSH6), so loss of one protein typically results in loss of its heterodimer partner [REC-B 6]. Loss of MLH1 expression in the presence of BRAF V600E mutation generally excludes Lynch syndrome diagnosis, though approximately 1% of BRAF V600E-mutant cancers with MLH1 loss still harbor Lynch syndrome [REC-B 5]. IHC testing looks at protein expression of all four MMR genes, and interpretation should report either "retained for all MMR proteins" or "loss of expression of (one or more of the MMR proteins)" [REC-B 6]. dMMR/MSI-H status is the primary determinant of eligibility for immune checkpoint inhibitor immunotherapy [REC-F 5]. Testing can be performed by IHC for protein expression, PCR for microsatellite instability, or as part of validated multigene panel testing [REC-B 5].

PI3K Pathway Alterations: Somatic PI3K pathway alterations include mutations in PIK3CA exons 9 and 20, other PIK3CA mutations, PIK3R1 mutations, PTEN mutations, and deep deletions of PTEN [REC-2 footnote m]. These alterations are clinically significant for stage II–III disease, as PIK3CA-mutant tumors benefit from adjuvant aspirin therapy (100–162 mg PO daily for 3 years after surgery). The ALASCCA trial reported hazard ratios for time to recurrence of 0.49 (95% CI, 0.24–0.98; P = .044) for PIK3CA exon 9/20 mutations and 0.42 (95% CI, 0.21–0.83; P = .013) for other PI3K pathway alterations with aspirin versus placebo [MS-30]. Molecular profiling to include somatic PI3K pathway alterations should be performed on all stage II and III colorectal cancer tumors [REC-3A].

POLE/POLD1 Proofreading Deficiency: Pathogenic variants in the exonuclease domains of POLE and POLD1 result in loss of DNA replication proofreading function, leading to ultra-hypermutated tumors with TMB exceeding 50 mutations per megabase [REC-B 7]. Somatic POLE mutations occur in approximately 2–8% of predominantly MSS/pMMR colorectal cancers, while somatic POLD1 mutations are extremely rare [REC-B 7]. Germline pathogenic variants predispose patients to polymerase proofreading-associated polyposis (PPAP) [REC-B 7]. These tumors exhibit enhanced immune responses due to numerous neoantigens and demonstrate favorable prognosis with excellent response to immune checkpoint inhibitor therapy [REC-B 7].

NTRK Gene Fusions: NTRK 1/2/3 gene fusions are extremely rare in colorectal cancers, occurring in approximately 0.35% of cases (8 of 2314 CRCs in one cohort) [REC-B 6]. These fusions are confined to tumors that are wild-type for KRAS, NRAS, and BRAF. Seven of eight CRCs harboring NTRK fusions were found in the dMMR/MSI-H subset [REC-B 6]. TRK inhibitors (larotrectinib, entrectinib, repotrectinib) demonstrate activity exclusively in tumors with NTRK gene fusions, not point mutations [REC-B 6]. RNA-based next-generation sequencing appears to be the optimal detection method, as it provides direct evidence of functional transcription [REC-B 6].

RET Gene Fusions: Activating RET gene fusions involving the C-terminal kinase domain lead to constitutive upregulation of RET kinase activity, promoting cell proliferation and survival through downstream MAPK and PI3K signaling pathways [REC-B 7]. The most common fusion partners reported in CRC include KIF5B, CCDC6, and NCOA4 [REC-B 7]. Selpercatinib is FDA-approved for RET fusion-positive solid tumors [REC-B 7]. Caution should be used in interpreting RET gene fusion results from ctDNA/RNA assays following targeted therapy with EGFR inhibitors [REC-B 7].

HER2 (ERBB2) Overexpression/Amplification: HER2 positivity is defined as IHC 3+ staining in >50% of tumor cells (intense membrane staining that can be circumferential, basolateral, or lateral) or amplification by FISH (HER2:CEP17 ratio ≥2 in >50% of cells) [REC-B 6]. Anti-HER2 therapies including pertuzumab or tucatinib combined with trastuzumab are indicated for HER2-overexpressing tumors that are KRAS/NRAS/BRAF wild-type [REC-F]. Fam-trastuzumab deruxtecan-nxki is indicated for HER2-overexpressing tumors with IHC 3+ [REC-F].

Tumor Budding: Defined as single cells or clusters of ≤4 neoplastic cells at the advancing edge of invasive carcinoma, tumor budding is graded as low (0–4 buds), intermediate (5–9 buds), or high (≥10 buds) per the International Tumor Budding Consensus Conference [REC-B 3]. High-grade tumor budding is an independent adverse prognostic factor for stage II colon cancer and is associated with increased risk of lymph node metastasis in pT1 cancers [REC-B 3]. A meta-analysis showed high-grade tumor budding increased the risk of poor outcomes in rectal cancer after neoadjuvant therapy (poor 5-year DFS, P < .00001; poor 5-year OS, P = .003; local recurrence, P = .007; and distant metastasis, P < .001) [MS-6].

Risk FactorsClick to collapse

Family history of colorectal cancer or adenomatous polyps

Approximately 20% of cases of colorectal cancer are associated with familial clustering. First-degree relatives of patients with colorectal adenomas or invasive CRC are at increased risk for colorectal cancer [MS-3]. The presence of a first-degree relative with CRC confers increased lifetime risk. All patients with rectal cancer should be counseled regarding family history [REC-1 footnote a].

Lynch syndrome (hereditary nonpolyposis colorectal cancer)

Lynch syndrome is caused by germline pathogenic variants in mismatch repair genes (MLH1, MSH2, MSH6, PMS2) and confers significantly elevated lifetime risk of colorectal cancer. Universal MMR or MSI testing is recommended in all newly diagnosed rectal cancer patients to identify potential Lynch syndrome [REC-B 5]. Loss of MLH1 expression in the setting of BRAF V600E mutation would preclude Lynch syndrome diagnosis in the vast majority of patients [REC-B 5]. The NCCN Guidelines for Genetic/Familial High-Risk Assessment: Colorectal, Endometrial, and Gastric provide detailed management recommendations [REC-1 footnote a].

Familial adenomatous polyposis (FAP) and attenuated FAP

FAP is characterized by development of hundreds to thousands of colorectal adenomatous polyps, with near-universal progression to colorectal cancer if untreated. Attenuated FAP presents with fewer polyps (typically 10–99) and later age of onset. All patients with rectal cancer should be counseled for family history, and those with suspected FAP or attenuated FAP should be referred for genetic risk assessment [REC-1 footnote a].

Age

Colorectal cancer incidence traditionally increases with age, though rising incidence has been documented in younger populations. The estimated incidence rate for rectal cancer in patients aged 20 to 34 years is projected to increase by 124.2% by 2030 [MS-2]. CRC that occurs in patients younger than 45 years may be clinicopathologically and genetically different from CRC in adults 45 years and older [MS-2]. The NCCN Guidelines recommend tools to aid optimal assessment and care of older adults with cancer [REC-2 footnote j].

Race and ethnicity (non-Hispanic Black populations)

Colorectal cancer incidence and mortality rates vary by race and ethnicity, with the highest rates in non-Hispanic Black individuals and the lowest in Asian Americans/Pacific Islanders [MS-2]. The magnitude of disparity in mortality rates is double that of incidence rates. Reasons include differences in risk factor prevalence, access to health care, social determinants of health, comorbidities, and tumor characteristics [MS-2].

Somatic PI3K pathway alterations (treatment risk modifier)

Tumors harboring somatic PI3K pathway alterations (PIK3CA exon 9 and 20 mutations, other PIK3CA mutations, PIK3R1 mutations, PTEN mutations, or deep deletions of PTEN) modify treatment recommendations. For stage II–III disease with these alterations, adjuvant aspirin is recommended, based on data showing reduced recurrence [REC-3A]. Molecular profiling to include PI3K pathway alterations should be done on all stage II and III CRC tumors [REC-3A].

Inflammatory bowel disease

The NCCN Rectal Cancer Guidelines reference the NCCN Guidelines for Colon Cancer for detailed discussion of risk factors including inflammatory bowel disease. Chronic colonic inflammation is an established risk factor for colorectal carcinogenesis. Patients with long-standing ulcerative colitis or Crohn's colitis require enhanced surveillance.

Clinical FeaturesClick to collapse

Typical Presentation

Rectal cancer commonly presents with changes in bowel habits including increased frequency, narrower stools, or a feeling of incomplete evacuation. Patients may report rectal bleeding (hematochezia), tenesmus (a constant feeling of needing to pass stools), abdominal or pelvic pain, and in advanced cases, bowel obstruction or perforation. Approximately 20% to 34% of patients with colorectal cancer present with synchronous liver metastases, and lung metastases occur in approximately 4% to 9% of patients. The rectum is defined as lying below a virtual line from the sacral promontory to the upper edge of the symphysis as determined by MRI [1,2]. Early-stage rectal cancers may be detected incidentally during screening colonoscopy or as malignant polyps (pT1 invasive adenocarcinomas). Malignant polyps are defined as those harboring cancer invading through the muscularis mucosae into the submucosa [3].

Symptoms

Common
Rectal bleeding

Hematochezia or occult blood in stool; one of the most common presenting symptoms

Common
Change in bowel habits

Increased frequency, narrower stools, diarrhea alternating with constipation

Common
Tenesmus

Constant feeling of incomplete evacuation or urgency to defecate

Common
Abdominal or pelvic pain

May be crampy or persistent; more common with advanced or obstructing tumors

Less common
Bowel obstruction

Partial or complete obstruction from advanced tumor; can present as acute emergency

Common in screened populations
Incidental detection on colonoscopy

Malignant polyps detected during screening or diagnostic colonoscopy; polyps may be pedunculated or sessile

Rare
Perforation

Spontaneous or secondary to tumor growth; medical emergency

Signs

Variable by stage
Palpable rectal mass

Identified on digital rectal examination (DRE); critical to document tumor size, distance from anal verge, orientation within rectal lumen, degree of circumferential involvement, extent of fixation, and sphincter involvement

Variable
Lymphadenopathy

Enlarged pelvic or inguinal lymph nodes on examination or imaging may indicate metastatic disease

Common
Fecal occult blood

Detectable on DRE or fecal immunochemical test

Variable
Obstructing lesion

May present with distended abdomen, tympany, or signs of bowel obstruction

Red FlagsClick to collapse

InvestigationsClick to collapse

Diagnostic

Colonoscopy

Essential for all patients with rectal cancer to evaluate for synchronous lesions, other polyps, or other pathologic conditions of the colon and rectum

Proctoscopy

Useful in determining distance of cancer from anal verge and length of tumor; may be performed at time of colonoscopy or within 2 weeks if appropriate

Endoscopic biopsy with pathology review

Confirm presence of invasive cancer (pT1); pTis has no biological potential to metastasize; careful pathology review for evidence of invasion through muscularis mucosa

Digital rectal examination (DRE)

Critical component of clinical assessment; should be performed by treating surgeon independently

Staging

Pelvis MRI with or without contrast

Preferred imaging for local staging of primary rectal tumor; assess T and N stage; predict CRM status; minimum requirement 1.5 T with external phased array coil; endorectal coils expressly NOT recommended

Endorectal ultrasound (EUS)

Alternative to MRI when MRI is contraindicated (e.g., pacemaker) or inconclusive, or for superficial (T1) lesions; limited for high or bulky tumors

Chest CT with IV contrast

Assess for distant metastatic disease to lungs, thoracic lymph nodes

Abdominal CT or MRI with IV contrast

Assess for liver metastases, peritoneal cavity, abdominal lymph nodes; MRI with hepatobiliary agent preferred for liver-directed therapy planning

FDG-PET/CT

Not routinely indicated for staging; may be considered for potentially surgically curable M1 disease in selected cases; does not supplant contrast-enhanced CT or MRI; may be necessary for equivocal findings

Biomarkers

Mismatch repair (MMR) or microsatellite instability (MSI) testing

Universal MMR or MSI testing recommended in ALL newly diagnosed rectal cancer patients; determines eligibility for checkpoint inhibitor immunotherapy; identifies Lynch syndrome

KRAS/NRAS and BRAF V600E mutation testing

Required for all patients with metastatic CRC; BRAF V600E mutation makes response to panitumumab or cetuximab highly unlikely; KRAS/NRAS mutations preclude EGFR inhibitor use

HER2 (ERBB2) overexpression/amplification testing

Required for patients with metastatic disease to determine eligibility for anti-HER2 therapy (trastuzumab-based regimens, fam-trastuzumab deruxtecan-nxki)

Molecular profiling to include somatic PI3K pathway alterations

Somatic PI3K pathway alterations include mutations in PIK3CA exon 9 and 20, other PIK3CA, PIK3R1, and PTEN mutations, and deep deletions of PTEN; if present, aspirin 100-162 mg PO daily for 3 years after recovery from surgery is recommended for stage II and III

Multigene panel testing (MGPT)

Should be conducted as part of MGPT to identify rare and actionable mutations and fusions such as POLE/POLD1, RET, and NTRK 1/2/3; may be tissue- or blood-based

Carcinoembryonic antigen (CEA)

Baseline and surveillance marker; elevated CEA may indicate metastatic disease; sensitivity 68% (95% CI, 53%-79%) and specificity 97% (95% CI, 90%-99%) at cutoff of 10 ng/mL

Complete blood count (CBC) and chemistry profile

Baseline laboratory assessment prior to treatment

DPYD testing

FDA Black Box warning to test patients for genetic variants of DPYD prior to initiating capecitabine unless immediate treatment is necessary

StagingClick to collapse

AJCC Cancer Staging Manual, 8th Edition (2017)

T Categories

StageDescription
TXPrimary tumor cannot be assessed
T0No evidence of primary tumor
TisCarcinoma in situ, intramucosal carcinoma (involvement of lamina propria with no extension through muscularis mucosae)
T1Tumor invades the submucosa (through the muscularis mucosae but not into the muscularis propria)
T2Tumor invades the muscularis propria
T3Tumor invades through the muscularis propria into pericolorectal tissues
T4Tumor invades the visceral peritoneum or invades or adheres to adjacent organ or structure
T4aTumor invades through the visceral peritoneum (including gross perforation of the bowel through tumor and continuous invasion through areas of inflammation to the surface of the visceral peritoneum)
T4bTumor directly invades or adheres to adjacent organs or structures

N Categories

StageDescription
NXRegional lymph nodes cannot be assessed
N0No regional lymph node metastasis
N1One to three regional lymph nodes are positive (tumor in lymph nodes measuring ≥0.2 mm), or any number of tumor deposits are present and all identifiable lymph nodes are negative
N1aOne regional lymph node is positive
N1bTwo or three regional lymph nodes are positive
N1cNo regional lymph nodes are positive, but there are tumor deposits in the subserosa, mesentery, or nonperitonealized pericolic, or perirectal/mesorectal tissues
N2Four or more regional lymph nodes are positive
N2aFour to six regional lymph nodes are positive
N2bSeven or more regional lymph nodes are positive

M Categories

StageDescription
M0No distant metastasis by imaging, etc.; no evidence of tumor in distant sites or organs
M1Metastasis to one or more distant sites or organs or peritoneal metastasis is identified
M1aMetastasis to one site or organ is identified without peritoneal metastasis
M1bMetastasis to two or more sites or organs is identified without peritoneal metastasis
M1cMetastasis to the peritoneal surface is identified alone or with other site or organ metastases

Stage Groupings

GroupCriteriaClinical MeaningFive Yr SurvivalTreatment Intent
Stage 0Tis, N0, M0Carcinoma in situ; confined to mucosa; no metastatic potentialNoneCurative
Stage IT1, T2, N0, M0Tumor invades submucosa or muscularis propria; no nodal involvementNoneCurative
Stage IIAT3, N0, M0Tumor penetrates through muscularis propria into pericolorectal tissues; node-negativeNoneCurative
Stage IIBT4a, N0, M0Tumor penetrates through visceral peritoneum; node-negativeNoneCurative
Stage IICT4b, N0, M0Tumor directly invades or adheres to adjacent organs/structures; node-negativeNoneCurative
Stage IIIAT1-T2, N1/N1c, M0 OR T1, N2a, M0Low T-stage with regional nodal involvementNoneCurative
Stage IIIBT3-T4a, N1/N1c, M0 OR T2-T3, N2a, M0 OR T1-T2, N2b, M0Higher T-stage with regional nodal involvement or lower T-stage with extensive nodal involvementNoneCurative
Stage IIICT4a, N2a, M0 OR T3-T4a, N2b, M0 OR T4b, N1-N2, M0Advanced local disease with extensive nodal involvementNoneCurative
Stage IVAAny T, Any N, M1aMetastasis to one site or organ without peritoneal metastasisNonePalliative with potential for cure in select resectable cases
Stage IVBAny T, Any N, M1bMetastasis to two or more sites or organs without peritoneal metastasisNonePalliative with potential for cure in select resectable cases
Stage IVCAny T, Any N, M1cPeritoneal metastasis with or without blood-borne metastasisNonePalliative

Staging Pearls

  • T stage has more prognostic value than N stage in rectal cancer; patients with stage IIIA (T1-T2) have longer rectal cancer-specific survival than patients with stage IIA (T3), IIB (T4a), and IIC (T4b) rectal cancer
  • Tumor deposits (N1c) are irregular discrete tumor deposits in perirectal fat away from the leading edge of the tumor showing no evidence of residual lymph node tissue; they are associated with reduced DFS and OS
  • A positive circumferential resection margin (CRM) is defined as tumor within 1 mm from the resected margin and is a strong predictor of local recurrence and OS
  • Patients with peritoneal carcinomatosis (M1c) have shorter PFS and OS than those without peritoneal involvement
  • The prefixes 'p' and 'yp' denote pathologic staging and pathologic staging following neoadjuvant therapy respectively
  • For stage II (pN0) disease, if <12 lymph nodes are initially identified, the pathologist should resubmit more tissue; lymph node retrieval is significantly reduced after neoadjuvant therapy (mean 13 vs 19, P < .05)
  • Isolated tumor clusters (ITC) measuring <0.2 mm are classified as N0 in the AJCC 8th edition staging system, while micrometastatic deposits measuring 0.2-2.0 mm are considered positive lymph nodes
  • Perineural invasion (PNI) is an independent prognostic factor; for stage II rectal cancer, those with PNI have significantly worse 5-year DFS compared to those without PNI (29% vs. 82%; P = .0005)
  • Rectal cancer has higher pelvic recurrence risk than colon cancer due to close proximity to pelvic structures, absence of serosa, and technical difficulties with wide surgical margins

SurveillanceClick to collapse

Clinical Follow Up Schedule

  • Low-risk polyps removed by polypectomy: Physical exam and proctoscopy every 3-6 months for 2 years. Colonoscopy at 1 year, then as per adenoma findings.
  • Transanal local excision only: Proctoscopy (with EUS or MRI) every 3-6 months for 2 years, then every 6 months for 5 years. Colonoscopy at 1 year.
  • Stage I with full surgical staging: Colonoscopy at 1 year.
  • Stage II-IV: History and physical exam every 3-6 months for 2 years, then every 6 months for 5 years. CEA every 3-6 months for 2 years, then every 6 months for 5 years (if candidate for curative-intent metastasectomy). Colonoscopy at 1 year, then as per adenoma findings.

Imaging Strategy

  • Stage II-III: Chest/abdomen/pelvis CT every 6-12 months for 5 years (category 2B for frequency <12 months).
  • Stage IV: C/A/P CT every 3-6 months for 2 years (category 2B for frequency <6 months), then every 6-12 months for 5 years.
  • After nonoperative management (watch-and-wait): MRI rectum every 6 months for up to 3 years. CT chest/abdomen every 6-12 months for 5 years.
  • FDG-PET/CT is usually not recommended for routine surveillance, but may be considered for equivocal findings or evaluation of isolated CEA elevation.

Laboratory Monitoring

  • CEA testing as per schedule above.
  • Routine blood counts and chemistry profile as needed for monitoring treatment effects and comorbidities.
  • ctDNA is not recommended for routine surveillance or treatment de-escalation outside of clinical trials.

Supportive Follow Up

  • Colonoscopy: At 1 year post-resection, then at 3 years, then every 5 years unless advanced adenoma found (repeat in 1 year).
  • Fertility counseling prior to treatment.
  • Smoking cessation counseling.
  • Screening for second primary cancers per age-appropriate guidelines.
  • Immunizations as indicated.
  • Referral to ostomy support group if applicable.

ComplicationsClick to collapse

Disease-Related

ComplicationManagement
Bowel obstruction or perforationMay require emergent surgical intervention, diverting ostomy, or stenting for palliation. Bevacizumab use in the setting of colon/rectal stents may increase the risk of perforation.
Anemia and nutritional deficiencySupportive care including iron supplementation, transfusion as needed, and nutritional support.
Local invasion causing fistulae or organ dysfunctionMultidisciplinary management including palliative radiation, chemotherapy, or surgery.

Supportive CareClick to collapse

Supportive care is integral throughout the treatment of rectal cancer, addressing physical, psychological, and social needs. It includes symptom management, nutritional support, and prevention/treatment of therapy-related toxicities. A multidisciplinary team approach is essential.

Nutritional Support

Assessment by a registered dietitian is recommended. Management may include oral nutritional supplements, dietary modification, and parenteral nutrition if necessary. Pelvic radiation can cause malabsorption and diarrhea; dietary manipulation and bulk-forming agents may help.

Anti Emetic Protocol

Follow anti-emetic guidelines based on the emetogenic potential of the chemotherapy regimen. 5-HT3 receptor antagonists and dexamethasone are commonly used. NK1 receptor antagonists may be added for highly emetogenic regimens.

Gcsf Guidance

Primary prophylaxis with granulocyte colony-stimulating factor (G-CSF) should be considered for regimens with a >20% risk of febrile neutropenia. Per NCCN guidelines for myeloid growth factors.

Vte Prophylaxis

Ambulation and mechanical prophylaxis are standard. Pharmacologic prophylaxis should be considered for high-risk patients, especially post-surgery, but careful risk-benefit assessment is needed given bleeding risk.

Pain Management

A multimodal approach is recommended. For oxaliplatin-induced neuropathy, duloxetine is recommended for painful neuropathy (not effective for numbness, tingling, or cold sensitivity). Pregabalin or gabapentin are not recommended. Referral to a pain management specialist for refractory cases.

Psychosocial Support

Screen for distress and provide resources (NCCN Guidelines for Distress Management). Address concerns about body image, sexuality, and fertility. Refer to social work, support groups, and survivorship programs as needed.

Dental Care

Good oral hygiene is important, especially during chemotherapy. A dental evaluation prior to treatment is recommended for patients requiring oral bisphosphonates or with poor dental health.

PrognosisClick to collapse

Colorectal cancer is the fourth most frequently diagnosed cancer and the second leading cause of cancer death in the United States. Approximately a third of colorectal cancers occur in the rectum. In 2025, an estimated 46,950 new cases of rectal cancer are expected in the US, with 52,900 deaths from rectal and colon cancers combined. Mortality rates have decreased by >50% from peak rates due to improved screening and treatment. [1]

By Stage

StageFive Yr SurvivalContext
Stage I (T1-2, N0, M0)NoneGenerally considered localized disease with a favorable prognosis when treated with surgery.
Stage II (T3-4, N0, M0)NonePrognosis varies based on specific pathologic features (e.g., T4 stage, lymphovascular invasion, perineural invasion).
Stage III (Any T, N1-2, M0)NoneNode-positive disease; prognosis is inversely correlated with the extent of nodal involvement (N1 vs. N2).
Stage IV (Any T, Any N, M1)NoneMetastatic disease. Prognosis depends on the extent of metastases (M1a, M1b, M1c) and resectability. Patients with peritoneal metastases (M1c) have a shorter progression-free and overall survival than those without peritoneal involvement. [2]

Prognostic Factors

  • Pathologic stage (T, N, M categories)
  • Circumferential resection margin (CRM) status (involved or threatened CRM defined as tumor within 1 mm of the resected margin)
  • Lymphovascular invasion (LVI)
  • Perineural invasion (PNI) - associated with significantly worse prognosis; an independent prognostic factor for cancer-specific, overall, and disease-free survival
  • Tumor deposits (irregular discrete tumor deposits in perirectal fat without residual lymph node tissue) - associated with reduced disease-free and overall survival
  • Tumor budding (single cells or clusters of ≤4 cells at the invasive edge) - high-grade budding is an adverse prognostic factor and may inform treatment decisions
  • Response to neoadjuvant therapy (tumor regression grade, ypT/ypN stage)
  • Microsatellite instability (MSI) or mismatch repair (MMR) status - dMMR/MSI-H tumors have a more favorable prognosis
  • POLE/POLD1 mutations - associated with an ultra-hypermutated phenotype (TMB >50 mut/Mb) and a more favorable prognosis, likely due to enhanced immune response

Follow UpClick to collapse

Post Curative Treatment

Follow-up is designed to monitor for recurrence, detect new metachronous neoplasms, and manage long-term sequelae. Intensity is based on stage and risk of recurrence. After curative-intent treatment, 95% of recurrences occur within the first 5 years. [3, 4]

Surveillance Rationale

Early detection of potentially resectable recurrent disease may allow for curative-intent salvage surgery. Surveillance also identifies metachronous polyps and second primary cancers at a pre-invasive stage. More intensive surveillance programs have been shown to detect recurrences earlier, but the impact on overall survival remains debated. [5, 6]

Late Effects Screening

  • Bowel function changes (chronic diarrhea, incontinence, urgency, clustering) - assess and manage with anti-diarrheal agents, bulk-forming agents, diet modification, pelvic floor rehabilitation.
  • Urogenital dysfunction (sexual dysfunction, erectile dysfunction, urinary incontinence, frequency, urgency) - screen and refer to urology/gynecology as needed.
  • Oxaliplatin-induced peripheral neuropathy - monitor symptoms; duloxetine for painful neuropathy.
  • Fatigue, insomnia, cognitive dysfunction - address with lifestyle modifications and referral if persistent.
  • Psychosocial distress - screen and provide support.
  • Potential for pelvic fractures/decreased bone density after pelvic radiation - consider bone density monitoring.

Recurrence Patterns

Most recurrences occur within the first 2-3 years. Local recurrence rates have been reduced with TME and neoadjuvant therapy. Distant metastases most commonly involve the liver and lungs. The pattern of recurrence (isolated local vs. distant) dictates further treatment options.

Key TrialsClick to collapse

AcronymFull NameYearNInterventionComparatorPopulationPrimary EndpointKey ResultSecondary OutcomesPractice ChangeJournal
RAPIDOShort-course radiotherapy followed by chemotherapy before total mesorectal excision versus preoperative chemoradiotherapy, TME, and optional adjuvant chemotherapy in locally advanced rectal cancer2021912Short-course RT (25 Gy/5 fractions) followed by CAPEOX or FOLFOX, then TME (experimental arm)Standard neoadjuvant long-course chemoRT (50.4 Gy with concurrent capecitabine or 5-FU), then TME, with optional adjuvant chemotherapyPatients with locally advanced rectal cancer (high-risk features)3-year disease-related treatment failure3-year disease-related treatment failure rate was 23.7% with TNT vs. 30.4% with standard treatment (HR 0.75; 95% CI 0.60-0.95; P = .019).Pathologic complete response (pCR) rate was 28% vs. 14%. No difference in overall survival. 5-year follow-up showed a higher locoregional recurrence rate with TNT (10% vs. 6%, P = .027).Established total neoadjuvant therapy (TNT) with short-course RT followed by chemotherapy as a standard of care for locally advanced rectal cancer, improving disease-free survival.Lancet Oncology
PRODIGE 23Neoadjuvant chemotherapy with FOLFIRINOX and preoperative chemoradiotherapy for patients with locally advanced rectal cancer2021461Neoadjuvant mFOLFIRINOX (6 cycles) followed by long-course chemoRT (50.4 Gy with capecitabine), then TMEStandard neoadjuvant long-course chemoRT, then TMEPatients with locally advanced rectal cancer (cT3-4, N+)3-year disease-free survival (DFS)3-year DFS was 76% in the TNT group vs. 69% in the standard group (HR 0.69; 95% CI 0.49-0.97; P = .034).7-year DFS 67.6% vs. 62.5%; 7-year OS 81.9% vs. 76.1%. Serious adverse events were lower in the TNT group (11% vs. 23%, P = .0049).Supported the use of intensive induction chemotherapy (FOLFIRINOX) as part of a TNT strategy for locally advanced rectal cancer, improving DFS.Lancet Oncology
PROSPECTPreoperative treatment of locally advanced rectal cancer20231128Neoadjuvant chemotherapy with mFOLFOX6 (12 cycles), with selective use of chemoRT if tumor regression ≤20%Neoadjuvant long-course chemoRT (50.4 Gy with concurrent 5-FU or capecitabine)Patients with stage II or III rectal cancer (cT2, N1-2 or cT3, N0-2) who were candidates for sphincter-sparing surgeryDisease-free survival at 3 yearsNoninferiority was established (HR 0.92; 95% CI 0.74-1.14; P = .005 for noninferiority). 5-year DFS was 80.8% with FOLFOX vs. 78.6% with chemoRT.Similar rates of locoregional recurrence, overall survival, and pathologic complete response. Patient-reported outcomes showed worse short-term AEs with chemoRT but worse long-term AEs (fatigue, neuropathy, sexual function) with FOLFOX.Demonstrated that selective omission of chemoRT after a favorable response to neoadjuvant chemotherapy is a viable option for patients meeting trial criteria, allowing avoidance of radiation-related toxicity.New England Journal of Medicine
BEACON CRCEncorafenib, binimetinib, and cetuximab in BRAF V600E-mutated colorectal cancer2019665Encorafenib + binimetinib + cetuximab (triplet) or encorafenib + cetuximab (doublet)Cetuximab + investigator's choice of irinotecan or FOLFIRIPatients with previously treated BRAF V600E-mutant metastatic CRCOverall survival (OS)Median OS was 9.0 months with triplet, 8.4 months with doublet, and 5.4 months with control (triplet vs. control HR 0.52; 95% CI 0.39-0.70; P < .001).Median progression-free survival (PFS) was 4.3 months (triplet), 3.2 months (doublet), and 1.5 months (control).Established encorafenib + cetuximab (with or without binimetinib) as a standard of care for previously treated BRAF V600E-mutant metastatic CRC.New England Journal of Medicine
KEYNOTE-177Pembrolizumab versus chemotherapy in microsatellite instability-high or mismatch repair-deficient metastatic colorectal cancer2020307Pembrolizumab (200 mg IV every 3 weeks)Investigator's choice of chemotherapy (FOLFOX or FOLFIRI) with or without bevacizumab or cetuximabPatients with previously untreated MSI-H/dMMR metastatic CRCProgression-free survival (PFS)Median PFS was 8.2 months with pembrolizumab vs. 5.9 months with chemotherapy (HR 0.60; 95% CI 0.45-0.80; P = .001).Objective response rate (ORR) 43.8% vs. 33.1%. 5-year OS rate was 53.9% vs. 35.5%.Established pembrolizumab as a first-line standard of care for MSI-H/dMMR metastatic CRC, improving PFS and offering durable responses.New England Journal of Medicine
CheckMate 142Nivolumab in patients with metastatic DNA mismatch repair-deficient/microsatellite instability-high colorectal cancer201774Nivolumab 3 mg/kg every 2 weeks ± ipilimumab 1 mg/kg every 3 weeks for 4 dosesNone (single-arm study)Patients with dMMR/MSI-H metastatic CRC (mostly previously treated)Objective response rate (ORR)ORR was 31% for nivolumab monotherapy and 55% for the combination. 12-month OS rate was 73%.Disease control rate 69% (monotherapy) and 79% (combination).Provided early evidence for the efficacy of nivolumab (± ipilimumab) in dMMR/MSI-H metastatic CRC, leading to FDA approval.Lancet Oncology

Clinical PearlsClick to collapse

  • Pearl 1: Multidisciplinary team evaluation is essential for all rectal cancer patients, particularly for treatment planning in locally advanced and metastatic disease.
  • Pearl 2: The rectum is defined as the segment of large bowel distal to the peritoneal reflection (from the sacral promontory to the upper edge of the symphysis pubis as determined by MRI).
  • Pearl 3: Circumferential resection margin (CRM) is a critical pathologic predictor of local recurrence; an involved or threatened CRM is defined as tumor within 1 mm of the resected margin.
  • Pearl 4: Total neoadjuvant therapy (TNT) is now the preferred approach for stage II-III rectal cancer, offering higher rates of pathologic complete response, improved treatment completion, and potential for organ preservation.
  • Pearl 5: For dMMR/MSI-H or POLE/POLD1-mutated tumors, neoadjuvant checkpoint inhibitor immunotherapy is the preferred first-line treatment for stage II-III disease, often leading to clinical complete response and avoidance of surgery.
  • Pearl 6: The addition of low-dose aspirin (100-162 mg daily for 3 years) after surgery is recommended for stage II-III tumors with somatic PI3K pathway alterations (PIK3CA mutations, PTEN loss).
  • Pearl 7: Nonoperative management (watch-and-wait) may be considered for patients with a clinical complete response after neoadjuvant therapy, but only at centers with experienced multidisciplinary teams and with the understanding of the increased risk of local regrowth, which is highest in the first 2-3 years.
  • Pearl 8: Surveillance after curative-intent treatment should be stage-stratified, with more intensive follow-up (CEA, imaging) for higher stages (II-IV) to detect potentially resectable recurrences.

Special SituationsClick to collapse

dMMR/MSI-H or POLE/POLD1 mutation with ultra-hypermutated phenotype (TMB >50 mut/Mb)
T1, N0 disease
Synchronous liver- or lung-only metastases
Unresectable metachronous metastases
Locally recurrent disease

Guidelines ResourcesClick to collapse

NCCN Clinical Practice Guidelines in Oncology: Rectal Cancer, Version 2.2026
NCCN Guidelines for Survivorship
NCCN Guidelines for Management of Immune Checkpoint Inhibitor-Related Toxicities
NCCN Guidelines for Genetic/Familial High-Risk Assessment: Colorectal, Endometrial, and Gastric
NCCN Guidelines for Distress Management
Colorectal Cancer Survivorship Care Guidelines

Protective FactorsClick to collapse

  • Cancer screening and early detection through polypectomy: The improvements in colorectal cancer incidence and mortality are thought to be a result of cancer prevention and earlier diagnoses through screening, as well as improved treatment modalities [MS-2]. Colonoscopy-based screening enables detection and removal of precancerous adenomatous polyps before malignant transformation, contributing to the overall decline in colorectal cancer incidence from 60.5 per 100,000 in 1976 to 38.7 per 100,000 in 2016 [MS-2].
  • Adjuvant aspirin for PIK3CA-mutated stage II–III disease: For stage II and III colorectal cancer tumors harboring somatic PI3K pathway alterations, daily aspirin (100–162 mg PO) for 3 years after recovery from surgery is recommended [REC-3A]. The ALASCCA trial reported hazard ratios for time to recurrence of 0.49 (95% CI, 0.24–0.98; P = .044) in Group A (PIK3CA exon 9/20 mutations) and 0.42 (95% CI, 0.21–0.83; P = .013) in Group B (other PI3K pathway alterations) with aspirin versus placebo [MS-30]. Aspirin should not be initiated until after recovery from surgery and can be given concurrently with adjuvant chemotherapy [REC-3A footnote m].
  • Neoadjuvant checkpoint inhibitor immunotherapy for dMMR/MSI-H or POLE/POLD1-mutated locally advanced disease: For stage II–III dMMR/MSI-H or POLE/POLD1-mutated rectal cancer, neoadjuvant immunotherapy with PD-1 inhibitors (dostarlimab-gxly, nivolumab, or pembrolizumab) is the preferred approach [REC-14]. Complete clinical response has been achieved in the majority of patients, enabling organ preservation without surgery, radiation, or chemotherapy [REC-14].